Novel fluorophore for labelling of oligonucleotides

One-step labelling of oligonucleotides with fluoresceine during automated synthesis.

Fluorescent or biotin labelling of an oligonucleotide at it's 5'-end usually involves two steps. First, a N-protected aminoalkyl phosphoramidite derivative (as for instance Aminolink 2 from Applied Biosystems) is added to the 5'-end of an oligonucleotide during automated DNA synthesis. After removal of all protecting groups the NHS ester of an appropriate fluorescent dye or biotin is coupled to...

Development of a Novel Fluorophore for Real-Time Biomonitoring System

Rapid in-field diagnosis is very important to prevent the outbreak of various infectious and contagious diseases. Highly sensitive and quantitative detection of diseases can be performed using fluorescent immunochemical assay with specific antigen-antibody binding and a good quality fluorophore. This can lead to the development of a small, portable, quantitative biosensor to transmit diagnostic...

Synthesis of oligonucleotides possessing versatile probes for PET labelling and their rapid ligand-free click reaction.

We synthesised aryl acetylene derivatives as versatile probes for labelling of oligonucleotides. RNA oligomers bearing an aryl acetylene molecule rapidly reacted with benzylazide derivatives under ligand-free click reaction conditions.

Polymerase synthesis of oligonucleotides containing a single chemically modified nucleobase for site-specific redox labelling.

Enzymatic construction of single-nucleobase redox-labelled oligonucleotides was developed either based on polymerase incorporation of a single modified nucleoside triphosphate (dNTP) followed by primer extension (PEX) with natural dNTPs or based on PEX with a biotinylated one-nucleotide overhang template, magnetoseparation and the second PEX with a full-length template.

Novel Methods for Synthesis of High Quality Oligonucleotides